Organism Tissue Morphology Culture Properties Homo sapiens (Human) Embryonic kidney Epithelial Adherent 本细胞株详细信息如下: General Information Cell Line Name 293 (Human Embryonic Kidney Cells) Synonyms HEK 293; HEK-293; 293 Organism Homo sapiens (Human) Tissue Embryonic kidney Cell Type － Morphology Epithelial Disease － Strain － Biosafety Level* 2 [Cells contain Adenovirus] Age at Sampling Fetus Gender － Genetics － Ethnicity － Applications Efficacy testing Category － * Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. Characteristics Karyotype This is a hypotriploid human cell line. The modal chromosome number was 64, occurring in 30% of cells. The rate of cells with higher ploidies was 4.2 %. The der(1)t(1;15) (q42;q13), der(19)t(3;19) (q12;q13), der(12)t(8;12) (q22;p13), and four other marker chromosomes were common to most cells. Five other markers occurred in some cells only. The marker der(1) and M8 (or Xq+) were often paired. There were four copies of N17 and N22. Noticeably in addition to three copies of X chromosomes, there were paired Xq+, and a single Xp+ in most cells. Virus Susceptibility － Derivation － Clinical Data － Antigen Expression － Receptor Expression Vitronectin, expressed Oncogene － Genes Expressed － Gene expression databases － Metastasis － Tumorigenic Yes Effects Yes, forms tumors in nude mice Comments Although an earlier report suggested that the cells contained Adenovirus 5 DNA from both the right and left ends of the viral genome, it is now clear that only left end sequences are present. The cells express an unusual cell surface receptor for vitronectin composed of the integrin beta－1 subunit and the vitronectin receptor alpha－v subunit. The Ad5 insert was cloned and sequenced, and it was determined that a colinear seqment from nts 1 to 4344 is integrated into chromosome 19 (19q13.2). Culture Method Doubling Time － Methods for Passages Wash by PBS once then 0.05% trypsin-EDTA solution and incubate at room temperature (or at 37ºC), observe cells under an inverted microscope until cell layer is dispersed (usually within 1 to 5 minutes) Medium 90% DMEM (high glucose)+10% FBS Special Remarks － Medium Renewal Every 2 to 3 days Subcultivation Ratio 1:6 to 1:10 weekly Growth Condition 95% air + 5% CO2, 37ºC Freeze medium DMEM (high glucose)+20% FBS+10% DMSO，也可以订购碧云天的细胞冻存液(C0210)。 本细胞株经过支原体检测(Mycoplasma Test)，检测结果为阴性。 本细胞株通过STR (short tandem repeats)鉴定，具体结果如下： AMEL D16S539 D7S820 D5S818 THO1 TPOX vWA D13S317 CSF1PO 本细胞株的培养图片如下：

保存条件：对于细胞培养瓶或离心管运输的活细胞，室温3-5天有效。对于干冰运输的冻存细胞，液氮保存，长期有效；-80ºC保存，2个月有效。

注意事项：
本细胞株未经碧云天书面许可不得用于任何商业用途，也不得移交给订货人所在实验室外的任何个人或单位。使用者在发表研究论文或结果时，应注明细胞株的来源。
本细胞株相关资料参考ATCC (American Type Culture Collection)、DSMZ (German Collection of Microorganisms and Cell Cultures)、JCRB (Japanese Collection of Research Bioresources Cell Bank)、Cellosaurus (Swiss Institute of Bioinformatics)等网站信息，并结合碧云天实际培养信息综合而成。由于细胞培养的条件、代数等因素，实际细胞可能与本说明书提供的信息有一定的差异，具体以实际细胞为准。
STR结果可以与ATCC、DSMZ及中国国家实验细胞资源共享平台等网站的数据库进行比对，匹配度80%以上即可认为该细胞系正确。
本产品会根据细胞是否正在培养、目的地距离等因素确定运输方式：冷冻的细胞冻存管(干冰)、一小瓶贴壁培养的细胞或一小瓶/管悬浮培养的细胞(常温)。为了更好地耐受长途运输和环境温度等变化，对于正常贴壁培养的细胞，也可能会以悬浮的形式培养在细胞培养瓶或离心管中进行运输。
对于干冰运输的冻存细胞，若干冰已经完全融化，请立即将细胞复苏培养，切勿再次低温冻存；若尚留有干冰，请直接复苏培养或立即将含有细胞的冻存管放入液氮中保存待用，切不可将细胞置于高温环境。
收到冻存的细胞后请尽快复苏细胞进行培养，以确认细胞活力、状态并保种。如暂时不进行复苏操作，冻存细胞可在-80ºC条件下保存2个月。
每支冻存管约含1×106个细胞，体积为0.5-1ml，预期存活率60-90%，建议复苏至1个6cm培养皿中。如果复苏后存活率较低，可以消化后转移至3.5cm培养皿中，这样细胞生长会更好。
如果本产品是常温运输，并且是培养瓶中充满完全培养液的贴壁细胞，收到细胞后请在显微镜下观察细胞生长状态，如果细胞密度超过85%请尽快进行传代操作；如果悬浮的细胞较多，请将培养瓶置于培养箱中静置过夜以使悬浮的细胞再次贴壁。如果收到的是常温运输的离心管装的悬浮细胞，可以直接取出转移至培养皿或培养瓶中培养。若培养液颜色正常则保留培养液继续培养，并且在首次更换培养液时，保